Description
Acute kidney injury (AKI) is associated with an increased risk of chronic kidney disease (CKD). To extend our understanding of renal repair, and its limits, we performed a detailed molecular characterization of a murine ischemia reperfusion injury (IRI) model for 12 months post injury. RNA-seq analysis highlights a cascade of temporal specific gene expression patterns related to tubular injury/repair, fibrosis, innate and adaptive immunity.
Overall Design
Murine renal bilateral ischemia reperfusion injury (IRI) was performed on 10- to 12-week-old (25- 28g) C57Bl/6NCrl male mice (Charles River). The mice were anesthetized with an intraperitoneal injection of a ketamine/xylazine mix (105 mg ketamine/kg; 10 mg xylazine/kg). The body temperature was maintained at 36.5~ 37C throughout the procedure. Kidneys were exposed by a midline abdominal incision and both renal pedicles were clamped for 21 min using non-traumatic microaneurysm clips. Sham-operated mice underwent the same procedure except for clamping of the pedicles. Cohorts of 3 to 4 biological replicates for injured, sham and uninjured kidneys were collected for RNA-seq at 10 different time points following IRI: 2, 4 hours; 1, 2, 3 days; 1, 2, 4 weeks; and 6, 12 months.
Curator
mj_li