Description
To investigate the mechanism of nephrotoxicity, colistin methanesulfonate sodium (CMS; 25 or 50 mg/kg) was administered via intraperitoneal injection to SpragueDawley rats daily over 7 d.; Affymetrix GeneChip microarrays indicated 894 differentially expressed genes in groups treated with CMS (ANOVA, FDR < 0.05, fold change 1.3).
Overall Design
The Affymetrix GeneChip Rat 230 2.0 (Affymetrix, Santa Clara, CA, USA) was used for microarray analysis. We randomly selected three rats from each CMS-treated group (0, 25, and 50 mg/kg). Microarray analysis was conducted according to the GeneChip 3’ Expression Arrays User Guide (Affymetrix). cDNA synthesis, cRNA synthesis, biotin labeling, cRNA purification, and fragmentation were performed according to the manufacturers instructions (Affymetrix). The microarrays were hybridized at 45C at 60 rpm for 16 h. The microarrays were stained and washed using a Fluidics Station 450 system (Affymetrix). A GeneChip Scanner 3000 (Affymetrix) was used to scan the microarrays. The reproducibility of the microarray experiments was confirmed using well-defined quality control criteria according to the manufacturers instructions.
Curator
hy_li