Description
High incidence of heart failure (HF) is a typical characteristic of chronic kidney disease (CKD). However, the pathogenesis of CKD-associated HF remains elusive. Here, we investigated the changes in myocardial energy metabolism in CKD mice and explored the underlying mechanisms.; To examine genome wide transcriptional changes in the heart of CKD mice, we performed microarray analysis using the Affymetrix Clariom S mouse.
Overall Design
To obtain the differentially expressed genes of the heart between sham and CKD mice. Mice were inflicted with 2/3 electrocoagulation of the right renal cortex and received left total nephrectomy 2 weeks later to construct CKD model. Mice underwent laparotomy without kidney operation served as sham group. Total RNA was extracted from the heart and reversely transcripted. Then, cDNA was hybridized using the Affymetrix Clariom S Mouse microarrays in duplicate.
Curator
hy_li