Description
The aim of this study was to identify the functional impact of Endothelial-to-Mesenchymal Transition (EndMT) in kidney fibrosis. For this purpose EndMT has been conditionally deleted in endothelial cells by the genetic deletion of Twist and Snail in the endothelial cells, using the tamoxifen inducible Cdh5-ERT2 Cre model. Twist EndMTcKO and Snail EndMTcKO mice, together with WT mice, were challanged with the unilateral ureteral obstruction model (UUO) to induce kidney fibrosis. Mice were euthanized 10 days after surgery. In addition, since we found that EndMT-induced vascular damage and hypoxia induces Myc upregulation in tubular epithelial cells (TECs), we deleted Myc in TECs (using the GGT-Cre model). Myc GGTcKO mice were also challenged with the UUO model and euthanized 10 days after surgery.
Overall Design
Transcriptional profile of fibrotic (UUO), healthy or contralateral kidneys from Twist, Snail and Myc conditional knock-out mice (triplicate for each experimental condition) was generated by whole transcriptome RNA-Seq using the Illumina platform.
Curator
xm_li