Description
Single-cell transcriptomics revealed that gluconeogenesis is impaired in proximal tubule cells during AKI in mice.
Overall Design
To investigate single cell transcriptomics on renal tubule cells, we generated a reporter mouse expressing GFP linked to the histone protein H2B in all nephron cells by combining 3 transgenes Six2-TGC; Rosa26rtTA ; pTRE-H2BeGFP. IRI and Sham surgeries were performed, and kidney tissue was harvested at different timepoints, followed by nuclei isolation, FACs sorting, and 10X Genomics Chromium Drop-Seq and library construction. Illumina Next-Seq sequencing of barcoded single nuclei libraries was performed and aligned to a pre-mrna genome with STAR/CellRanger. Gene/barcode matrices were merged and processed with the Seurat R package.
Curator
xm_li