Description
Tertiary lymphoid tissues (TLTs) are formed in systemic organs manifesting chronic inflammation. Herein, we found that the renal pelvis (RP) could form urinary tract-associated lymphoid tissues (UTALTs) in a TLT-formation manner in humans and mice with chronic kidney disease (CKD), regardless of infectious pyelonephritis. Furthermore, collagen type XVII alpha 1 chain (COL17A1), localized ectopically to the transitional epithelium (TE) covering the UTALT, where it participated in TE development via immunological stimulation of UTALT-forming cells.
Overall Design
The spleens were isolated from C57BL/6N at 6-month old, minced, washed, resuspended, and cultured. These cultured cells were stimulated with or without recombinant human COL17A1 for 3 hours. And then, RNA was collected and gene expression was analyzed.
Curator
xm_li