Description
Mincle (Macrophage inducible C-type lectin) is a pattern recognition receptor expressed in innate immune cells and can sense cell death, suggesting a role in sterile inflammation. We induced renal ischemiareperfusion injury to KO and WT mice and collected the kidneys to compare the gene expression.; Microarray analysis revealed that the overlap between the genes upregulated in injured kidney vs. sham kidney in WT mice and the genes downregulated in injured kidney in KO mice vs. WT mice included inflammation-related pathways on day 3.
Overall Design
We induced renal ischemiareperfusion injury and collected kidneys at day 1 and day 3 for RNA extraction and hybridization on Affymetrix microarrays.
Curator
xm_li