RGED RGED / PARP1 binds and regulates expression of extracellular matrix genes in renal tubular epithelial cells [RIP-Seq]

Public on 2020-09-06

Description

To exploit the potential function of PARP1 during renal IRI by binding target RNA to regulate its expression, we obtained PARP1 bound peaks in proximal tubular epithelial cell (HK-2) by iRIP-seq for the first time, which showed that the bind peaks of PARP1_IP group is more enriched at GGUAA-rich motifs and PARP1 preferentially bound to the downstream of TSS (transcription start site) and upstream of TTS (transcription termination site). iRIP-seq analysis was used to decipher RBP binding sites and revealed the RNA that interacts with PARP1 within the genome of HK-2 cells.

Overall Design

RIP-seq analysis of PARP1 IP versus input in HK-2 cells

Curator

xm_li

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