Description
Single-cell transcriptomics revealed that gluconeogenesis is impaired in proximal tubule cells during CKD in mice.
Overall Design
Normal mouse kidney was analysed by single-nucleus RNA-sequencing using 10X Genomics Chromium Drop-Seq. Normal kidney was harvested from 10- to 12-week-old, 2528-g, male C57BL6/J Pten pc-/-. Illumina Next-Seq sequencing of barcoded single nuclei libraries was performed and aligned to a pre-mrna genome with STAR/CellRanger. Seurat v3.2.0 in R v4 was used for downstream analyses, including normalization, scaling, and clustering of nuclei.
Curator
xm_li