Description
We use tolDCs from syngeneic (C57BL6) and allogeneic (BALB/c) mice to show potent renoprotective abilities in a ischemia reperfusion AKI model. Dendritic cells were derived from mice bone marrow and cultured for 7 days with GM-CSF and IL-4. Tolerogenic DCs were cultured in the same DC media but with the addition of VitD3 and IL10. LPS was added on day 6 and cells harvested for use or analysis on day 7.
Overall Design
C57BL6 mice bone marrow derived dendritic cells (BMDC) and VitD3/IL10 generated tolerogenic (tolDC) cells were sequenced with or without LPS stimulation (n = 3 per group)
Curator
yq_pan